Monoclonal Anti-TCEB3 antibody produced in mouse
SIGMA/WH0006924M2 - clone 1F3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-SIII; Anti-TCEB3A; Anti-transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to TCEB3 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting TCEB3 monoclonal antibody, clone 1F3 Western Blot analysis of TCEB3 expression in HeLa S3 NE.
Western Blotting Western Blot analysis of TCEB3 expression in transfected 293T cell line by TCEB3 monoclonal antibody, clone 1F3. Lanes Lane 1: TCEB3 transfected lysate (87.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_003198   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL |
| General description: | This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. (provided by RefSeq) |
| Immunogen: | TCEB3 (NP_003189, 81 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence NAEPDEQDFEKSNSRKRPRDALQKE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |