Monoclonal Anti-TCF8 antibody produced in mouse
SIGMA/WH0006935M1 - clone 4C4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-AREB6; Anti-BZP; Anti-NIL2A; Anti-PPCD3; Anti-ZEB; Anti-ZEB1; Anti-ZFHEP; Anti-ZFHX1A; Anti-transcription factor 8 (represses interleukin 2 expression)
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to ZEB1 on HeLa cell. [antibody concentration 10 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to ZEB1 on U-2 OS cell. [antibody concentration 10 μg/mL]
Immunofluorescence U-2 OS cells were stained with ZEB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged ZEB1 is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_030751   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P37275 |
| General description: | ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM |
| Immunogen: | ZEB1 (NP_110378, 801 a.a. ~ 900 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence NIAIPTVTAQLPTIVAIADQNSVPC |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |