Monoclonal Anti-TCF12 antibody produced in mouse
SIGMA/WH0006938M1 - clone 2E9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HEB; Anti-HTF4; Anti-HsT17266; Anti-transcription factor 12 (HTF4, helix-loop-helix transcription factors 4)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TCF12 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 μg/mL]
Western Blotting Western Blot analysis of TCF12 expression in transfected 293T cell line by TCF12 monoclonal antibody, clone 2E9. Lanes Lane 1: TCF12 transfected lysate (75.8 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of TCF12 over-expressed 293 cell line, cotransfected with TCF12 Validated Chimera RNAi. Blot probed with TCF12 monoclonal antibody, clone 2E9. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting TCF12 monoclonal antibody, clone 2E9 Western Blot analysis of TCF12 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (35.64 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_207036   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q99081 |
| General description: | The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. (provided by RefSeq) |
| Immunogen: | TCF12 (NP_996919, 364 a.a. ~ 453 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VGSPSPLTGTSQWPRPGGQAPSSPS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |