Monoclonal Anti-PRDX2 antibody produced in mouse
SIGMA/WH0007001M1 - clone 4E10-2D2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC4104; Anti-NKEFB; Anti-PRP; Anti-PRXII; Anti-Peroxiredoxin 2; Anti-TDPX1; Anti-TSA
MDL Number: MFCD00162725
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PRDX2 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to PRDX2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PRDX2 expression in transfected 293T cell line by PRDX2 monoclonal antibody, clone 4E10-2D2. Lanes Lane 1: PRDX2 transfected lysate (21.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting PRDX2 monoclonal antibody, clone 4E10-2D2 Western Blot analysis of PRDX2 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (47.52 kDa).
ELISA Detection limit for recombinant GST tagged PRDX2 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E10-2D2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC000452   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P32119 |
| Application: | Monoclonal Anti-PRDX2 antibody produced in mouse has been used in western blotting (1:1000). |
| Biochem/physiol Actions: | Peroxiredoxin 2 (PRDX2) effectively neutralizes hydrogen peroxide (H2O2 ). It is regarded as a critical cell survival modulator and may serve as a key regulator for cell proliferation and intracellular signaling. Downregulated expression of PRDX2 is observed in acute myeloid leukemia (AML) and melanoma. However, it acts as an oncogene in the pathophysiology of various cancers including non-small cell lung cancer (NSCLC), esophageal, cervical, gastric tumors, and many more. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Peroxiredoxin 2 (PRDX2) is an antioxidant protein that belongs to the peroxiredoxins family. It is associated with mammalian erythrocytes. The PRDX2 gene is mapped to human chromosome19p13.13. |
| Immunogen: | PRDX2 (AAH00452, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MASGNARIGKPAPDFKATAVVDGAF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |