Monoclonal Anti-TEAD4 antibody produced in mouse
SIGMA/WH0007004M1 - clone 5H3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-EFTR2; Anti-MGC9014; Anti-RTEF1; Anti-TCF13L1; Anti-TEA domain family member 4; Anti-TEF3; Anti-TEFR1; Anti-hRTEF1B
Product Type: Chemical
Western Blotting JOURNAL CITATION: Hippo/YAP signaling pathway is involved in osteosarcoma chemoresistance. By: Wang, D. Y., Wu, Y. N., et al. in Chin J Cancer, 2016. PubMed ID: 27206784 Image collected and cropped by CiteAb from the following publication, (https://cancercommun.biomedcentral.com/articles/10.1186/s40880-016-0109-z), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting Western Blot analysis of TEAD4 expression in transfected 293T cell line by TEAD4 monoclonal antibody, clone 5H3. Lanes Lane 1: TEAD4 transfected lysate (34.2 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of TEAD4 over-expressed 293 cell line, cotransfected with TEAD4 Validated Chimera RNAi. Blot probed with TEAD4 monoclonal antibody, clone 5H3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting TEAD4 monoclonal antibody, clone 5H3 Western Blot analysis of TEAD4 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
Immunoprecipitation Immunoprecipitation of TEAD4 transfected lysate using anti-TEAD4 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with TEAD4 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged TEAD4 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5H3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_003213   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q62296 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. (provided by RefSeq) |
| Immunogen: | TEAD4 (NP_003204, 151 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ARGPGRPAVSGFWQGALPGQAGTSH |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |