Monoclonal Anti-TFF3 antibody produced in mouse
SIGMA/WH0007033M1 - clone 3D9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HITF; Anti-ITF; Anti-TFI; Anti-hP1.B; Anti-trefoil factor 3 (intestinal)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TFF3 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Western Blotting QC Western Blot detection against Immunogen (32.23 kDa).
ELISA Detection limit for recombinant GST tagged TFF3 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC017859   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q07654 |
| Application: | Monoclonal Anti-TFF3 antibody produced in mouse has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Trefoil factor-3 (TFF-3) may be useful as a molecular marker for certain types of cancer, but its role in tumorigenesis is unknown. TFF3 promotes airway epithelial cell migration and differentiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. (provided by RefSeq) They are secreted peptides that are protease-resistant. Trefoil factor-3 (TFF-3) is expressed in goblet cells. |
| Immunogen: | TFF3 (AAH17859.1, 15 a.a. ~ 73 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EEYVGLSANQCAVPAKDRVDCGYPH |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |