Monoclonal Anti-TMEM1 antibody produced in mouse
SIGMA/WH0007109M1 - clone 5B4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-EHOC1; Anti-GT334; Anti-MGC126777; Anti-transmembrane protein 1
Product Type: Chemical
Western Blotting Western Blot analysis of TMEM1 expression in transfected 293T cell line by TMEM1 monoclonal antibody, clone 5B4. Lanes Lane 1: TMEM1 transfected lysate (142.2 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of TMEM1 over-expressed 293 cell line, cotransfected with TMEM1 Validated Chimera RNAi. Blot probed with TMEM1 monoclonal antibody, clone 5B4. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting TMEM1 monoclonal antibody, clone 5B4. Western Blot analysis of TRAPPC10 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (36.3 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5B4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_003274   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P48553 |
| General description: | The protein encoded by this gene is a transmembrane protein found in the cis-Golgi complex. The encoded protein is part of the multisubunit transport protein particle (TRAPP) complex and may be involved in vesicular transport from the endoplasmic reticulum to the Golgi. Mutations in this gene could be responsible for the Unverricht-Lundborg type of progressive myoclonus epilepsy, or for autoimmune polyglandular disease type 1. (provided by RefSeq) |
| Immunogen: | TMEM1 (NP_003265, 1162 a.a. ~ 1257 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VRLFKYLPHHSAHSSQLDADSWIEN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |