Monoclonal Anti-TNP1 antibody produced in mouse
SIGMA/WH0007141M1 - clone 1B5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TP1; Anti-transition protein 1 (during histone to protamine replacement)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TNP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 μg/mL]
Western Blotting QC Western Blot detection against Immunogen (31.79 kDa).
ELISA Detection limit for recombinant GST tagged TNP1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC029516   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P09430 |
| General description: | Transition protein-1 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines (see PRM1, MIM 182880; PRM2, MIM 182890) (Luerssen et al., 1990 [PubMed 2249851]).[supplied by OMIM |
| Immunogen: | TNP1 (AAH29516.1, 1 a.a. ~ 55 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSTSRKLKSHGMRRSKSRSPHKGVK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |