Monoclonal Anti-TOP2A antibody produced in mouse
SIGMA/WH0007153M1 - clone 1E2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TOP2; Anti-TP2A; Anti-topoisomerase (DNA) II alpha 170kDa
Product Type: Chemical
antibody form | purified immunoglobulin |
antibody product type | primary antibodies |
biological source | mouse |
clone | 1E2, monoclonal |
conjugate | unconjugated |
form | buffered aqueous solution |
GenBank® accession no. | NM_001067 ![]() ![]() |
isotype | IgG1κ |
Quality Level | 100 ![]() |
shipped in | dry ice |
species reactivity | human |
storage temp. | −20°C |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
indirect ELISA: suitable | |
indirect immunofluorescence: suitable | |
western blot: 1-5 μg/mL | |
UniProt accession no. | P11388 ![]() ![]() |
Biochem/physiol Actions: | Topoisomerase II A (TOP2A) enzyme plays a vital role in DNA replication and cell proliferation. Experimental studies show an increased expression of this enzyme in Chinese patients with gastric carcinoma. DNA topoisomerase 2-α acts as a target enzyme for an anthracycline drug called as epirubicin and many other antineoplastic drugs. Top2α is involved in reducing the topological stress in cells. The hsa-miR-485-3p downregulates the expression of nuclear transcription factor Y subunit β (NFYB), which acts as a mediator of Top2α. Consequently, it decreases the expression of Top2α and its drug responsiveness. Top2α enzyme plays an important role in maintenance of chromosome condensation and segregation. Ataxia telangiectasia mutated (ATM)-dependent regulation of TOP2A helps in TOP2 stability and also it’s sensitivity to TOP2 inhibitor. |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. (provided by RefSeq) |
Immunogen: | TOP2A (NP_001058, 1435 a.a. ~ 1531 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RAAPKGTKRDPALNSGVSQKPDPAK |
Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
Physical form: | Solution in phosphate buffered saline, pH 7.4 |
RIDADR | NONH for all modes of transport |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352203 |