Monoclonal Anti-NR2C2 antibody produced in mouse
SIGMA/WH0007182M1 - clone 2A5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TAK1; Anti-TR2R1; Anti-TR4; Anti-hTAK1; Anti-nuclear receptor subfamily 2, group C, member 2
MDL Number: MFCD02097469
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to NR2C2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 μg/mL]
Enhanced Validation-RNAi Western blot analysis of NR2C2 over-expressed 293 cell line, cotransfected with NR2C2 Validated Chimera RNAi. Blot probed with NR2C2 monoclonal antibody, clone 2A5. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of NR2C2 expression in transfected 293T cell line by NR2C2 monoclonal antibody, clone 2A5. Lanes Lane 1: NR2C2 transfected lysate (65.414 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_003298   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P49116 |
| General description: | Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al., 1996 [PubMed 8661150]).[supplied by OMIM |
| Immunogen: | NR2C2 (NP_003289, 43 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KIVTDQQTGQKIQIVTAVDASGSPK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |