Monoclonal Anti-TSTA3 antibody produced in mouse
SIGMA/WH0007264M1 - clone 2B9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FX; Anti-P35B; Anti-tissue specific transplantation antigen P35B
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of TSTA3 over-expressed 293 cell line, cotransfected with TSTA3 Validated Chimera RNAi. Blot probed with TSTA3 monoclonal antibody, clone 2B9. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting TSTA3 monoclonal antibody, clone 2B9 Western Blot analysis of TSTA3 expression in HeLa.
Western Blotting Western Blot analysis of TSTA3 expression in transfected 293T cell line by TSTA3 monoclonal antibody, clone 2B9. Lanes Lane 1: TSTA3 transfected lysate (35.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged TSTA3 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_003313   |
| isotype | IgG3κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13630 |
| General description: | Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymanno |
| Immunogen: | TSTA3 (NP_003304, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence DLAQLFIWVLREYNEVEPIILSVGE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |