Monoclonal Anti-TTN antibody produced in mouse
SIGMA/WH0007273M2 - clone 2B3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CMD1G; Anti-CMH9; Anti-CMPD4; Anti-DKFZp451N061; Anti-FLJ32040; Anti-LGMD2J; Anti-TMD; Anti-titin
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TTN on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 1 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to TTN on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged TTN is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC058824   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8WZ42 |
| Biochem/physiol Actions: | Titin (TTN) is an elastic protein that maintains cardiac muscle elasticity. S-glutathionylation of cryptic cysteines inhibits protein folding as well as disrupts mechanical stability that further increases titin elasticity through posttranslational modifications. TTN controls the temporal and spatial organizations of the striated muscle. During myofibrillogenesis, it phosphorylates the muscle protein telethonin for differentiating myocytes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | TTN (Titin) is the giant muscle protein consisting of a catalytic serine kinase domain. The gene encoding this protein is localized on human chromosome 2q31.2. |
| Immunogen: | TTN (AAH58824, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTTQAPTFTQPLQSVVVLEGSTATF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |