Monoclonal Anti-TWIST1 antibody produced in mouse
SIGMA/WH0007291M1 - clone 3E11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ACS3; Anti-BPES2; Anti-BPES3; Anti-SCS; Anti-TWIST; Anti-twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila)
MDL Number: MFCD08705531
Product Type: Chemical
Western Blotting Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody, clone 3E11. Lanes Lane 1: TWIST1 transfected lysate (21 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of TWIST1 over-expressed 293 cell line, cotransfected with TWIST1 Validated Chimera RNAi. Blot probed with TWIST1 monoclonal antibody, clone 3E11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting TWIST1 monoclonal antibody, clone 3E11. Western Blot analysis of TWIST1 expression in rat testis.
Western Blotting QC Western Blot detection against Immunogen (37.07 kDa).
Immunoprecipitation Immunoprecipitation of TWIST1 transfected lysate using anti-TWIST1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with TWIST1 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_000474   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15672 |
| Biochem/physiol Actions: | Twist family bHLH transcription factor 1 (TWIST1) has a role in the determination of cell lineage and differentiation during embryogenesis. During the development of neural crest, it is also involved in modulating cell movement and tissue reorganization. Mutations in the TWIST1 gene have been associated with Saethre-Chotzen syndrome (4) and the protein is also linked to various cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Twist family bHLH transcription factor 1 (TWIST1) is a basic helix-loop-helix transcription factor and the gene encoding it is localized on human chromosome 7p21.1. |
| Immunogen: | TWIST1 (NP_000465, 100 a.a. ~ 202 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PQSYEELQTQRVMANVRERQRTQSL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |