Monoclonal Anti-TXN antibody produced in mouse
SIGMA/WH0007295M1 - clone 2A7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC61975; Anti-TRX; Anti-thioredoxin
MDL Number: MFCD00282491
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to TXN on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of TXN expression in transfected 293T cell line by TXN monoclonal antibody, clone 2A7. Lanes Lane 1: TXN transfected lysate (11.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting TXN monoclonal antibody, clone 2A7 Western Blot analysis of TXN expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.29 kDa).
Immunoprecipitation Immunoprecipitation of TXN transfected lysate using anti-TXN monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with TXN rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged TXN is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC003377   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P10599 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It is ubiquitous and found in many organisms from plants and bacteria to mammals. Multiple in vitro substrates for thioredoxin have been identified, including ribonuclease, choriogonadotropins, coagulation factors, glucocorticoid receptor, and insulin. Reduction of insulin is classically used as an activity test.[supplied by OMIM |
| Immunogen: | TXN (AAH03377, 1 a.a. ~ 105 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MVKQIESKTAFQEALDAAGDKLVVV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |