Monoclonal Anti-TYRO3 antibody produced in mouse
SIGMA/WH0007301M5 - clone 4F6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BYK; Anti-Brt; Anti-Dtk; Anti-RSE; Anti-Sky; Anti-TYRO3 protein tyrosine kinase; Anti-Tif
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of TYRO3 over-expressed 293 cell line, cotransfected with TYRO3 Validated Chimera RNAi. Blot probed with TYRO3 monoclonal antibody (M05) clone 4F6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of TYRO3 expression in transfected 293T cell line by TYRO3 monoclonal antibody, clone 4F6. Lanes Lane 1: TYRO3 transfected lysate (96.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.52 kDa).
ELISA Detection limit for recombinant GST tagged TYRO3 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC049368   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q06418 |
| Immunogen: | TYRO3 (AAH49368, 50 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VKLTVSQGQPVRLNCSVEGMEEPDI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |