Monoclonal Anti-UBE2H antibody produced in mouse
SIGMA/WH0007328M1 - clone 3C4-1A2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-E220K; Anti-UBC8; Anti-UBCH; Anti-UBCH2; Anti-
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting UBE2H monoclonal antibody, clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
Western Blotting Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody, clone 3C4-1A2. Lanes Lane 1: UBE2H transfected lysate (20.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (45.87 kDa).
ELISA Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C4-1A2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC006277   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P62256 |
| General description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. (provided by RefSeq) |
| Immunogen: | UBE2H (AAH06277, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSSPSPGKRRMDTDVVKLIESKHEV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |