Monoclonal Anti-UCHL1 antibody produced in mouse
SIGMA/WH0007345M1 - clone 1B8-4D2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-PARK5; Anti-PGP9.5; Anti-ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to UCHL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ~ 10 μg/mL]
Western Blotting Western Blot analysis of UCHL1 expression in transfected 293T cell line by UCHL1 monoclonal antibody, clone 1B8-4D2. Lanes Lane 1: UCHL1 transfected lysate (Predicted MW: 24.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting UCHL1 monoclonal antibody, clone 1B8-4D2 Western Blot analysis of UCHL1 expression in IMR-32.
Western Blotting QC Western Blot detection against Immunogen (50.27 kDa).
ELISA Detection limit for recombinant GST tagged UCHL1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B8-4D2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC000332   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P09936 |
| Biochem/physiol Actions: | Ubiquitin C-terminal hydrolase L1 (UCHL1) catalyzes the hydrolysis of carboxy-terminal ubiquitin esters and amides. It plays an essential role in epithelial-mesenchymal transition. In addition, UCHL1 is also implicated in the cell invasion and in the cell cycle regulation. UCHL1 gene acts as an oncogene in various types of cancers including glioma, gastric cardiac adenocarcinoma (GCA) and colorectal cancer. It also acts as a susceptibility gene for Parkinson′s disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease |
| General description: | Ubiquitin C-terminal hydrolase L1 (UCHL1) is encoded by the gene mapped to human chromosome 4p14. The encoded protein is a deubiquitinase, which is part of the ubiquitin proteasome system (UPS). UCHL1 is specifically expressed in the nervous system and gonads. |
| Immunogen: | UCHL1 (AAH00332, 1 a.a. ~ 223 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MQLKPMEINPEMLNKVLSRLGVAGQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |