Monoclonal Anti-USF2 antibody produced in mouse
SIGMA/WH0007392M1 - clone 5E9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FIP; Anti-upstream transcription factor 2, c-fos interacting
MDL Number: MFCD01864392
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting USF2 monoclonal antibody, clone 5E9. Western Blot analysis of USF2 expression in HeLa.
Western Blotting USF2 monoclonal antibody, clone 5E9. Western Blot analysis of USF2 expression in PC-12.
Western Blotting Western Blot analysis of USF2 expression in transfected 293T cell line by USF2 monoclonal antibody, clone 5E9. Lanes Lane 1: USF2 transfected lysate (37 kDa). Lane 2: Non-transfected lysate.
Western Blotting USF2 monoclonal antibody, clone 5E9. Western Blot analysis of USF2 expression in HeLa S3 NE.
Western Blotting USF2 monoclonal antibody, clone 5E9. Western Blot analysis of USF2 expression in different cell lines.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged USF2 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5E9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_003367   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15853 |
| General description: | This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene. (provided by RefSeq) |
| Immunogen: | USF2 (NP_003358, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDMLDPGLDPAASATAAAAASHDKG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |