Monoclonal Anti-VDR antibody produced in mouse
SIGMA/WH0007421M2 - clone 2F4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-NR1I1; Anti-vitamin D (1,25- dihydroxyvitamin D3) receptor
MDL Number: MFCD00802899
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to VDR on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 μg/mL]
ELISA Detection limit for recombinant GST tagged VDR is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC060832   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| UniProt accession no. | P11473 |
| Biochem/physiol Actions: | VDR (vitamin D receptor) binds with its ligand 1a, 25-dihydroxyvitamin D3 [1,25(OH)2D3] or vitamin D3, and act upon the target cell nuclei to induce various biological effects. Vitamin D3 is also thought to have immunomodulatory effects, and gene polymorphisms are linked with pertussis. This variant might thus, affect the immune response and clinical outcome of Bordetella pertussis infection. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | VDR (vitamin D receptor) or vitamin D3 receptor is a member of the steroid and thyroid hormone receptors superfamily of trans-acting transcriptional regulatory factors. It is an intracellular polypeptide of 50-60kDa. This receptor is composed of a highly conserved cysteine, lysine, and arginine-rich DNA-binding domain, and a hydrophobic ligand-binding domain in its C-terminal. It shares size and sequence similarity to thyroid hormone receptor. VDR gene is localized to human chromosome 12q12–q14. |
| Immunogen: | VDR (AAH60832, 1 a.a. ~ 427 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEAMAASTSLPDPGDFDRNVPRICG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |