Monoclonal Anti-VHL antibody produced in mouse
SIGMA/WH0007428M1 - clone 1G12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HRCA1; Anti-RCA1; Anti-VHL1; Anti-von Hippel-Lindau tumor suppressor
MDL Number: MFCD01634357
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to VHL on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of VHL expression in transfected 293T cell line by VHL monoclonal antibody, clone 1G12. Lanes Lane 1: VHL transfected lysate (19.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (38.21 kDa).
ELISA Detection limit for recombinant GST tagged VHL is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_000551   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P40337 |
| General description: | Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. (provided by RefSeq) |
| Immunogen: | VHL (NP_000542, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPRRAENWDEAEVGAEEAGVEEYGP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |