Monoclonal Anti-VIM antibody produced in mouse
SIGMA/WH0007431M1 - clone 3E9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ36605; Anti-vimentin
MDL Number: MFCD00145922
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to VIM on U-2 OS cell. [antibody concentration 10 μg/mL]
Immunofluorescence A-549 cells were stained with VIM-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
Immunofluorescence Immunofluorescence of monoclonal antibody to VIM on HeLa cell. [antibody concentration 40 μg/mL]
Western Blotting Western Blot analysis of VIM expression in transfected 293T cell line by VIM monoclonal antibody, clone 3E9. Lanes Lane 1: VIM transfected lysate (53.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting VIM monoclonal antibody, clone 3E9 Western Blot analysis of VIM expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of VIM transfected lysate using anti-VIM monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with VIM rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC030573   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P08670 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract |
| Immunogen: | VIM (AAH30573, 41 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GSALRPSTSRSLYASSPGGVYATRS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |