Monoclonal Anti-YY1 antibody produced in mouse
SIGMA/WH0007528M1 - clone 2C4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DELTA; Anti-NFE1; Anti-UCRBP; Anti-YINYANG1; Anti-YY1 transcription factor
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of YY1 expression in transfected 293T cell line by YY1 monoclonal antibody, clone 2C4. Lanes Lane 1: YY1 transfected lysate (44.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting YY1 monoclonal antibody, clone 2C4 Western Blot analysis of YY1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged YY1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_003403   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P25490 |
| Application: | Monoclonal Anti-YY1 antibody produced in mouse has been used in immunohistochemistry. |
| Biochem/physiol Actions: | YY1 (Yin Yang 1) is a transcriptional repressor protein encoded by the YY1 gene in humans. It regulates diverse biological processes and may have an important role in carcinogenesis. Its expression is found to be upregulated in gastric cancer cell lines and primary gastric cancers. YY1 is found to contribute to carcinogenesis in gastric cancer. This DNA-binding protein is found to regulate the activity of the c-fos promoter. YY1 has roles in cell proliferation, differentiation, apoptosis and cell cycle progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene YY1 (Yin Yang 1) encodes a protein belonging to the GLI-Krüppel gene family. It is a zinc finger protein that is ubiquitously expressed. The YY1 gene is mapped to human chromosome 14q32. |
| Immunogen: | YY1 (NP_003394, 221 a.a. ~ 320 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VTMWSSDEKKDIDHETVVEEQIIGE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |