Monoclonal Anti-SF1 antibody produced in mouse
SIGMA/WH0007536M1 - clone 2E12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-D11S636; Anti-ZFM1; Anti-ZNF162; Anti-splicing factor 1
MDL Number: MFCD01634201
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SF1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody, clone 2E12. Lanes Lane 1: SF1 transfected lysate (Predicted MW: 59.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting SF1 monoclonal antibody, clone 2E12. Western Blot analysis of SF1 expression in NIH/3T3.
Western Blotting SF1 monoclonal antibody, clone 2E12. Western Blot analysis of SF1 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
Immunoprecipitation Immunoprecipitation of SF1 transfected lysate using anti-SF1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with SF1 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged SF1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_004630   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15637 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | SF1 (NP_004621, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MATGANATPLDFPSKKRKRSRWNQD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |