Monoclonal Anti-ZBTB16 antibody produced in mouse
SIGMA/WH0007704M1 - clone 3A7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-PLZF; Anti-ZNF145; Anti-zinc finger and BTB domain containing 16
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between TRAF2 and ZBTB16. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ZBTB16 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of ZBTB16 expression in transfected 293T cell line by ZBTB16 monoclonal antibody, clone 3A7. Lanes Lane 1: ZBTB16 transfected lysate (Predicted MW: 11.11 kDa). Lane 2: Non-transfected lysate.
ELISA Detection limit for recombinant GST tagged ZBTB16 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC029812   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q05516 |
| General description: | This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. (provided by RefSeq) |
| Immunogen: | ZBTB16 (AAH29812, 381 a.a. ~ 480 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QRELFSKLGELAVGMKSESRTIGEQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |