Monoclonal Anti-ZYX antibody produced in mouse
SIGMA/WH0007791M1 - clone 2C10-4A7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ESP2; Anti-HED2; Anti-zyxin
MDL Number: MFCD01634364
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to ZYX on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody, clone 2C10-4A7. Lanes Lane 1: ZYX transfected lysate (61.3 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi. Blot probed with ZYX monoclonal antibody, clone 2C10-4A7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (88.66 kDa).
Immunoprecipitation Immunoprecipitation of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with ZYX rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged ZYX is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C10-4A7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC008743   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15942 |
| General description: | Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. (provided by RefSeq) |
| Immunogen: | ZYX (AAH08743, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAAPRPSPAISVSVSAPAFYAPQEK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |