Monoclonal Anti-MAPKAPK3 antibody produced in mouse
SIGMA/WH0007867M1 - clone 3F4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-3PK; Anti-MAPKAP3; Anti-
MDL Number: MFCD01633617
Product Type: Chemical
Western Blotting Western Blot analysis of MAPKAPK3 expression in transfected 293T cell line by MAPKAPK3 monoclonal antibody, clone 3F4. Lanes Lane 1: MAPKAPK3 transfected lysate (Predicted MW: 43 kDa). Lane 2: Non-transfected lysate.
Western Blotting MAPKAPK3 monoclonal antibody, clone 3F4. Western Blot analysis of MAPKAPK3 expression in NIH/3T3.
Western Blotting MAPKAPK3 monoclonal antibody, clone 3F4 Western Blot analysis of MAPKAPK3 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
Immunoprecipitation Immunoprecipitation of MAPKAPK3 transfected lysate using anti-MAPKAPK3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MAPKAPK3 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged MAPKAPK3 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC001662   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q3UMW7 |
| General description: | This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. (provided by RefSeq) |
| Immunogen: | MAPKAPK3 (AAH01662, 272 a.a. ~ 382 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EVSEDAKQLIRLLLKTDPTERLTIT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |