Monoclonal Anti-ARHGEF5 antibody produced in mouse
SIGMA/WH0007984M1 - clone 3A12-B5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp686N1969; Anti-GEF5; Anti-P60; Anti-Rho guanine nucleotide exchange factor (GEF) 5; Anti-TIM; Anti-TIM1
Product Type: Chemical
Western Blotting Western Blot analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody, clone 3A12-B5. Lanes Lane 1: ARHGEF5 transfected lysate (60.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (82.83 kDa).
ELISA Detection limit for recombinant GST tagged ARHGEF5 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A12-B5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC014555   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q12774 |
| General description: | Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. (provided by RefSeq) |
| Immunogen: | ARHGEF5 (AAH14555, 1 a.a. ~ 519 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGGFSRRCSKLINSSQLLYQEYSDV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |