Monoclonal Anti-CLPP antibody produced in mouse
SIGMA/WH0008192M1 - clone 300, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
Product Type: Chemical
Western Blotting JOURNAL CITATION: Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals. By: Kühl, I., Miranda, M., et al. in Elife, 2017. PubMed ID: 29132502 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/30952), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CLPP on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1 μg/mL]
Western Blotting CLPP monoclonal antibody, clone 3E2 Western Blot analysis of CLPP expression in A-431.
Western Blotting CLPP monoclonal antibody, clone 3E2. Western Blot analysis of CLPP expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged CLPP is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 300, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_006012   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q16740 |
| Application: | Monoclonal Anti-CLPP antibody produced in mouse has been used in Western Blotting. |
| Biochem/physiol Actions: | CLPP (caseinolytic mitochondrial matrix peptidase proteolytic subunit) gene encodes a protease component of the Clp complex that hydrolyzes peptides and various proteins in the presence of ATP. The protein requires magnesium for its activity. CLPP is found to be associated with the inner mitochondrial membrane. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CLPP (caseinolytic mitochondrial matrix peptidase proteolytic subunit) protein contains an aqueous chamber formed by face-to-face assembly of the two heptameric rings that have proteolytic active sites within. It is a member of the peptidase family S14. The CLPP gene is localized to human chromosome 19p13.3. |
| Immunogen: | CLPP (NP_006003, 178 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence HQPSGGARGQATDIAIQAEEIMKLK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |