Monoclonal Anti-NR0B2 antibody produced in mouse
SIGMA/WH0008431M1 - clone 1A11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-SHP; Anti-SHP1; Anti-nuclear receptor subfamily 0, group B, member 2
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NR0B2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting NR0B2 monoclonal antibody, clone 1A11. Western Blot analysis of NR0B2 expression in human colon.
ELISA Detection limit for recombinant GST tagged NR0B2 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC030207   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15466 |
| General description: | The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. (provided by RefSeq) |
| Immunogen: | NR0B2 (AAH30207, 1 a.a. ~ 257 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSTSQPGACPCQGAASRPAILYALL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |