Monoclonal Anti-IKBKG antibody produced in mouse
SIGMA/WH0008517M1 - clone 4G9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FIP3; Anti-Fip3p; Anti-IKKgamma; Anti-IP; Anti-IP2; Anti-NEMO; Anti-inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
MDL Number: MFCD02263389
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to IKBKG on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to IKBKG on HeLa cell. [antibody concentration 8 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between RALBP1 and IKBKG. HeLa cells were stained with anti-RALBP1 rabbit purified polyclonal 1:1200 and anti-IKBKG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting IKBKG monoclonal antibody, clone 4G9 Western Blot analysis of IKBKG expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (71.83 kDa).
ELISA Detection limit for recombinant GST tagged IKBKG is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4G9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC050612   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9Y6K9 |
| General description: | This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [supplied by RefSeq |
| Immunogen: | IKBKG (AAH50612, 1 a.a. ~ 419 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MNRHLWKSQLCEMVQPSGGPAADQD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |