Monoclonal Anti-DGKE antibody produced in mouse
SIGMA/WH0008526M3 - clone 7E1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DAGK6; Anti-DGK; Anti-diacylglycerol kinase, epsilon 64kDa
Product Type: Chemical
Western Blotting DGKE monoclonal antibody, clone 7E1. Western Blot analysis of DGKE expression in NIH/3T3.
Western Blotting DGKE monoclonal antibody, clone 7E1 Western Blot analysis of DGKE expression in HeLa.
Western Blotting DGKE monoclonal antibody, clone 7E1. Western Blot analysis of DGKE expression in PC-12.
Western Blotting DGKE monoclonal antibody, clone 7E1. Western Blot analysis of DGKE expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged DGKE is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7E1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_003647   |
| isotype | IgG3λ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P52429 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. (provided by RefSeq) |
| Immunogen: | DGKE (NP_003638, 141 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence CKQQCGCQPKLCDYRCIWCQKTVHD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |