Monoclonal Anti-CDC14A antibody produced in mouse
SIGMA/WH0008556M1 - clone 2C12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CDC14 cell division cycle 14 homolog A (S. cerevisiae); Anti-Cdc14A1; Anti-Cdc14A2; Anti-cdc14; Anti-hCDC14
MDL Number: MFCD04975116
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to CDC14A on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of CDC14A expression in transfected 293T cell line by CDC14A monoclonal antibody, clone 2C12. Lanes Lane 1: CDC14A transfected lysate (66.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged CDC14A is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_003672   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9UNH5 |
| General description: | The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. (provided by RefSeq) |
| Immunogen: | CDC14A (NP_003663, 431 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PFRLSSSLQGSAVTLKTSKMALSPS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |