Monoclonal Anti-PRKRA antibody produced in mouse
SIGMA/WH0008575M1 - clone 1B9-1A7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HSD14; Anti-PACT; Anti-RAX; Anti-protein kinase, interferon-inducible double stranded RNA dependent activator
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5 μg/mL]
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. [antibody concentration 5 μg/mL]
Western Blotting PRKRA monoclonal antibody, clone 1B9-1A7 Western Blot analysis of PRKRA expression in HeLa.
Western Blotting Western Blot analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody, clone 1B9-1A7. Lanes Lane 1: PRKRA transfected lysate (34.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting PRKRA monoclonal antibody, clone 1B9-1A7. Western Blot analysis of PRKRA expression in human liver.
Western Blotting QC Western Blot detection against Immunogen (60.17 kDa).
Immunoprecipitation Immunoprecipitation of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PRKRA rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PRKRA is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B9-1A7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC009470   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O75569 |
| General description: | This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. (provided by RefSeq) |
| Immunogen: | PRKRA (AAH09470, 1 a.a. ~ 313 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSQSRHRAEAPPLEREDSGTFSLGK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |