Monoclonal Anti-DDX3Y antibody produced in mouse
SIGMA/WH0008653M1 - clone 2D7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DBY; Anti-DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to DDX3Y on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 μg/mL]
Western Blotting DDX3Y monoclonal antibody, clone 2D7 Western Blot analysis of DDX3Y expression in HeLa.
Western Blotting DDX3Y monoclonal antibody, clone 2D7. Western Blot analysis of DDX3Y expression in NIH/3T3.
Western Blotting DDX3Y monoclonal antibody, clone 2D7. Western Blot analysis of DDX3Y expression in Raw 264.7.
Western Blotting DDX3Y monoclonal antibody, clone 2D7. Western Blot analysis of DDX3Y expression in mouse testis.
Western Blotting QC Western Blot detection against Immunogen (34.54 kDa).
ELISA Detection limit for recombinant GST tagged DDX3Y is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_004660   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O15523 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified. (provided by RefSeq) |
| Immunogen: | DDX3Y (NP_004651, 1 a.a. ~ 80 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSHVVVKNDPELDQQLANLDLNSEK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |