Monoclonal Anti-TNKS antibody produced in mouse
SIGMA/WH0008658M1 - clone 1A2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-PARP5A; Anti-PARP5a; Anti-PARPL; Anti-TIN1; Anti-TINF1; Anti-TNKS1; Anti-tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
MDL Number: MFCD02686643
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TNKS on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 μg/mL]
Western Blotting QC Western Blot detection against Immunogen (37.62 kDa).
ELISA Detection limit for recombinant GST tagged TNKS is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_003747   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O95271 |
| Biochem/physiol Actions: | TNKS (tankyrase) is a regulator of Wnt-signaling. It negatively regulates Axin1, thereby suppressing β-catenin degradation and causing hyperactivation of Wnt/β-catenin signaling. TNKS controls cell death and telomere length. It might also be associated with osteoarthritis. In murine lung cancer model, silencing of this gene reduces lung cancer cell growth. Application of TNKS inhibitor suppresses growth of hepatocellular carcinoma cells. Absence of TNKS activity helps in replication of human cytomegalovirus (HCMV). |
| General description: | The gene TNKS (tankyrase) is mapped to human chromosome 8p23.1. It belongs to the PARP (poly (ADP-ribose) polymerase) family of proteins and is responsible for addition of poly (ADP-ribose) (PAR) to substrate proteins. The TNKS protein contains ankyrin repeats and a sterile α-motif (SAM). |
| Immunogen: | TNKS (NP_003738, 1017 a.a. ~ 1124 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TERKEGEVAGLDMNISQFLKSLGLE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |