Monoclonal Anti-RIOK3 antibody produced in mouse
SIGMA/WH0008780M1 - clone 4A12-1D2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-RIO kinase 3 (yeast); Anti-SUDD
Product Type: Chemical
Western Blotting RIOK3 monoclonal antibody, clone 4A12-1D2. Western Blot analysis of RIOK3 expression in MCF-7.
Western Blotting Western Blot analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody, clone 4A12-1D2. Lanes Lane 1: RIOK3 transfected lysate (59.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (82.5 kDa).
Immunoprecipitation Immunoprecipitation of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with RIOK3 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged RIOK3 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A12-1D2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC039729   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O14730 |
| General description: | This gene was identified by the similarity of its product to the Aspergillus nidulans SUDD protein, an extragenic suppressor of the heat-sensitive bimD6 mutation that fails to attach properly to the spindle microtubules at a restrictive temperature. The specific function of this gene has not yet been determined. (provided by RefSeq) |
| Immunogen: | RIOK3 (AAH39729, 1 a.a. ~ 516 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDLVGVASPEPGTAAAWGPSKCPWA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |