Monoclonal Anti-PCAF antibody produced in mouse
SIGMA/WH0008850M2 - clone 1H2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CAF; Anti-GCN5; Anti-GCN5L; Anti-GCN5L1; Anti-P/CAF; Anti-
MDL Number: MFCD03791929
Product Type: Chemical
Western Blotting PCAF monoclonal antibody, clone 1H2. Western Blot analysis of PCAF expression in LNCaP.
Western Blotting Western Blot analysis of PCAF expression in transfected 293T cell line by PCAF monoclonal antibody, clone 1H2. Lanes Lane 1: PCAF transfected lysate (93 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of PCAF over-expressed 293 cell line, cotransfected with PCAF Validated Chimera RNAi. Blot probed with PCAF monoclonal antibody, clone 1H2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting PCAF monoclonal antibody, clone 1H2 Western Blot analysis of PCAF expression in HeLa S3 NE.
Western Blotting PCAF monoclonal antibody, clone 1H2. Western Blot analysis of PCAF expression in human uterus myoma.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC060823   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q92831 |
| General description: | CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. (provided by RefSeq) |
| Immunogen: | PCAF (AAH60823, 353 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LEEEVYSQNSPIWDQDFLSASSRTS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |