Monoclonal Anti-EIF2B2 antibody produced in mouse
SIGMA/WH0008892M1 - clone 5B12-E10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-EIF2B; Anti-EIF2Bβ; Anti-Eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to EIF2B2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 5 μg/mL]
Western Blotting EIF2B2 monoclonal antibody, clone 5B12-E10 Western Blot analysis of EIF2B2 expression in K-562.
Western Blotting EIF2B2 monoclonal antibody, clone 5B12-E10. Western Blot analysis of EIF2B2 expression in HeLa.
Western Blotting Western Blot analysis of EIF2B2 expression in transfected 293T cell line by EIF2B2 monoclonal antibody, clone 5B12-E10. Lanes Lane 1: EIF2B2 transfected lysate (39 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (64.35 kDa).
ELISA Detection limit for recombinant GST tagged EIF2B2 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5B12-E10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC000494   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P49770 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon (EIF2B5; MIM 603945) subunits. EIF2B activates its EIF2 (see MIM 603907) substrate by exchanging EIF2-bound GDP for GTP.[supplied by OMIM |
| Immunogen: | EIF2B2 (AAH00494, 1 a.a. ~ 351 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPGSAAKGSELSERIESFVETLKRG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |