Monoclonal Anti-CPNE1 antibody produced in mouse
SIGMA/WH0008904M1 - clone 8B8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-COPN1; Anti-CPN1; Anti-MGC1142; Anti-copine I
Product Type: Chemical
Western Blotting Western Blot analysis of CPNE1 expression in transfected 293T cell line by CPNE1 monoclonal antibody, clone 8B8. Lanes Lane 1: CPNE1 transfected lysate (59.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting CPNE1 monoclonal antibody, clone 8B8 Western Blot analysis of CPNE1 expression in HeLa.
Enhanced Validation-RNAi Western blot analysis of CPNE1 over-expressed 293 cell line, cotransfected with CPNE1 Validated Chimera RNAi. Blot probed with CPNE1 monoclonal antibody, clone 8B8. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged CPNE1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8B8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_003915   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q99829 |
| General description: | Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins. (provided by RefSeq) |
| Immunogen: | CPNE1 (NP_003906, 111 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TLPLMLKPGKPAGRGTITVSAQELK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |