Monoclonal Anti-UBE2M antibody produced in mouse
SIGMA/WH0009040M1 - clone 3C4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-UBC12; Anti-UBCRS2; Anti-hUbc12; Anti-
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to UBE2M on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting UBE2M monoclonal antibody, clone 3C4 Western Blot analysis of UBE2M expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (45.87 kDa).
ELISA Detection limit for recombinant GST tagged UBE2M is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC058924   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P61081 |
| General description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. (provided by RefSeq) |
| Immunogen: | UBE2M (AAH58924, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MIKLFSLKQQKKEEESAGGTKGSSK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |