Monoclonal Anti-PAPSS2 antibody produced in mouse
SIGMA/WH0009060M7 - clone 2A8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-3′-phosphoadenosine 5′-phosphosulfate synthase 2; Anti-ATPSK2; Anti-SK2
MDL Number: MFCD02097223
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PAPSS2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to PAPSS2 on A-431 cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PAPSS2 expression in transfected 293T cell line by PAPSS2 monoclonal antibody, clone 2A8. Lanes Lane 1: PAPSS2 transfected lysate (69.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting PAPSS2 monoclonal antibody, clone 2A8. Western Blot analysis of PAPSS2 expression in NIH/3T3.
Western Blotting PAPSS2 monoclonal antibody, clone 2A8 Western Blot analysis of PAPSS2 expression in A-431.
Enhanced Validation-RNAi Western blot analysis of PAPSS2 over-expressed 293 cell line, cotransfected with PAPSS2 Validated Chimera RNAi. Blot probed with PAPSS2 monoclonal antibody, clone 2A8. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged PAPSS2 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_004670   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O95340 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3′-phosphoadenosine 5′-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. (provided by RefSeq) |
| Immunogen: | PAPSS2 (NP_004661, 513 a.a. ~ 612 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence DPAGMPHPETKKDLYEPTHGGKVLS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |