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Monoclonal Anti-PAPSS1 antibody produced in mouse

SIGMA/WH0009061M5 - clone 1F4, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-3′-phosphoadenosine 5′-phosphosulfate synthase 1; Anti-ATPSK1; Anti-PAPSS; Anti-SK1

MDL Number: MFCD06798511
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-WH0009061M5-100UG 100 µg
 $541.00
1/EA		 Add To Favorites
Immunofluorescence Immunofluorescence of monoclonal antibody to PAPSS1 on HeLa cell. [antibody concentration 10 μg/mL]
Enhanced Validation-RNAi Western blot analysis of PAPSS1 over-expressed 293 cell line, cotransfected with PAPSS1 Validated Chimera RNAi. Blot probed with PAPSS1 monoclonal antibody, clone 1F4. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of PAPSS1 expression in transfected 293T cell line by PAPSS1 monoclonal antibody, clone 1F4. Lanes Lane 1: PAPSS1 transfected lysate (68.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone 1F4, monoclonal
conjugate unconjugated
form buffered aqueous solution
GenBank accession no. NM_005443 
isotype IgG2aκ
Quality Level 100 
shipped in dry ice
species reactivity human
storage temp. −20°C
target post-translational modification unmodified
technique(s) immunofluorescence: suitable
  indirect ELISA: suitable
  western blot: 1-5 μg/mL
UniProt accession no. O43252 
General description: Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM
Immunogen: PAPSS1 (NP_005434, 144 a.a. ~ 252 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
QIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAE
Legal Information: GenBank is a registered trademark of United States Department of Health and Human Services
Physical form: Solution in phosphate buffered saline, pH 7.4
RIDADR NONH for all modes of transport
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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