Monoclonal Anti-CLDN1 antibody produced in mouse
SIGMA/WH0009076M1 - clone 1C5-D9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CLD1; Anti-ILVASC; Anti-SEMP1; Anti-claudin 1
MDL Number: MFCD02096101
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 5 μg/mL]
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Western Blotting CLDN1 monoclonal antibody, clone 1C5-D9. Western Blot analysis of CLDN1 expression in PRC/PRF/5.
Western Blotting Western Blot analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody, clone 1C5-D9. Lanes Lane 1: CLDN1 transfected lysate (23 kDa). Lane 2: Non-transfected lysate.
Western Blotting CLDN1 monoclonal antibody, clone 1C5-D9. Western Blot analysis of CLDN1 expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (48.95 kDa).
Immunoprecipitation Immunoprecipitation of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with CLDN1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged CLDN1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C5-D9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC012471   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O95832 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. (provided by RefSeq) |
| Immunogen: | CLDN1 (AAH12471.1, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MANAGLQLLGFILAFLGWIGAIVST |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |