Monoclonal Anti-USP14 antibody produced in mouse
SIGMA/WH0009097M4 - clone 6D6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TGT; Anti-ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to USP14 on HeLa cell. [antibody concentration 20 μg/mL]
Western Blotting USP14 monoclonal antibody, clone 6D6. Western Blot analysis of USP14 expression in PC-12.
Western Blotting USP14 monoclonal antibody, clone 6D6. Western Blot analysis of USP14 expression in Raw 264.7.
Western Blotting Western Blot analysis of USP14 expression in transfected 293T cell line by USP14 monoclonal antibody, clone 6D6. Lanes Lane 1: USP14 transfected lysate (56.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting USP14 monoclonal antibody, clone 6D6 Western Blot analysis of USP14 expression in HeLa.
Western Blotting USP14 monoclonal antibody, clone 6D6. Western Blot analysis of USP14 expression in NIH/3T3.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged USP14 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6D6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005151   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P54578 |
| Biochem/physiol Actions: | Ubiquitin specific peptidase 14 (USP14) releases ubiquitin from the proteasome-targeted ubiquitinated proteins resulting in regeneration of ubiquitin at the proteasome. It is involved in the degradation of chemokine receptor CXCR4. Under endoplasmic reticulum (ER) stress, USp14 binds to ER stress receptor, which is involved in unfolded protein response (UPR), a mechanism to control the accumulation of unfolded proteins within the ER lumen. Its overexpression leads to ER-associated degradation (ERAD) pathway inhibition. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ubiquitin specific peptidase 14 (USP14) is a 494 amino acid protein containing a 45kDa catalytic domain at its amino-terminus. Its ubiquitin-like (Ubl) domain associates with 19S regulatory particle (RP) 26S proteasome. This binding activates the deubiquitinating activity of the protein. USP14 is part of the ubiquitin-specific processing (UBP) family of proteases. |
| Immunogen: | USP14 (NP_005142, 395 a.a. ~ 494 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PQKEVKYEPFSFADDIGSNNCGYYD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |