Monoclonal Anti-ARHGEF1 antibody produced in mouse
SIGMA/WH0009138M3 - clone 4C4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-GEF1; Anti-LBCL2; Anti-P115RHOGEF; Anti-Rho guanine nucleotide exchange factor (GEF) 1; Anti-SUB1.5
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to ARHGEF1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting ARHGEF1 monoclonal antibody, clone 4C4 Western Blot analysis of ARHGEF1 expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (36.41 kDa).
ELISA Detection limit for recombinant GST tagged ARHGEF1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC034013   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q92888 |
| General description: | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. (provided by RefSeq) |
| Immunogen: | ARHGEF1 (AAH34013.2, 830 a.a. ~ 927 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence CRPGPEGQLAATALRKVLSLKQLLF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |