Monoclonal Anti-ZBED1 antibody produced in mouse
SIGMA/WH0009189M1 - clone 5G1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ALTE; Anti-KIAA0785; Anti-TRAMP; Anti-zinc finger, BED-type containing 1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to ZBED1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting ZBED1 monoclonal antibody, clone 5G1 Western Blot analysis of ZBED1 expression in HeLa S3 NE.
Western Blotting Western Blot analysis of ZBED1 expression in transfected 293T cell line by ZBED1 monoclonal antibody, clone 5G1. Lanes Lane 1: ZBED1 transfected lysate (78.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.41 kDa).
ELISA Detection limit for recombinant GST tagged ZBED1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5G1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_004729   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O96006 |
| Biochem/physiol Actions: | Zinc finger, BED-type containing 1 (ZBED1) function as a crucial transcription factor for cell proliferation, and is implicated in cell cycle-dependent regulation of a number of ribosomal protein (RP) genes. The encoded protein has been suggested to be involved in the transposition of other transposable elements. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Zinc finger, BED-type containing 1 (ZBED1), also known as DNA replication-related element-binding factor (DREF), is encoded by the single-exon gene mapped to Xp/Yp PAR region that has a functional homolog on the Y chromosome and escapes X-inactivation. |
| Immunogen: | ZBED1 (NP_004720, 3 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence NKSLESSQTDLKLVAHPRAKSKVWK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |