Monoclonal Anti-STK17A antibody produced in mouse
SIGMA/WH0009263M3 - clone 4D12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DRAK1; Anti-
MDL Number: MFCD02686100
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to STK17A on formalin-fixed paraffin-embedded human colon cancer. [antibody concentration 1.2 μg/mL]
Western Blotting Western Blot analysis of STK17A expression in transfected 293T cell line by STK17A monoclonal antibody, clone 4D12. Lanes Lane 1: STK17A transfected lysate (46.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting STK17A monoclonal antibody, clone 4D12 Western Blot analysis of STK17A expression in HepG2.
Enhanced Validation-RNAi Western blot analysis of STK17A over-expressed 293 cell line, cotransfected with STK17A Validated Chimera RNAi. Blot probed with STK17A monoclonal antibody (M03) clone 4D12. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (38.54 kDa).
ELISA Detection limit for recombinant GST tagged STK17A is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4D12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC047696   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9UEE5 |
| General description: | This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. (provided by RefSeq) |
| Immunogen: | STK17A (AAH47696, 301 a.a. ~ 413 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LLVKKPEDRATAEECLKHPWLTQSS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |