Monoclonal Anti-UBE4A antibody produced in mouse
SIGMA/WH0009354M8 - clone 1G8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-E4; Anti-KIAA0126; Anti-MGC133315; Anti-UBOX2; Anti-UFD2; Anti-ubiquitination factor E4A (UFD2 homolog, yeast)
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to UBE4A on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting UBE4A monoclonal antibody, clone 1G8. Western Blot analysis of UBE4A expression in PC-12.
Western Blotting UBE4A monoclonal antibody, clone 1G8. Western Blot analysis of UBE4A expression in NIH/3T3.
Western Blotting UBE4A monoclonal antibody, clone 1G8. Western Blot analysis of UBE4A expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged UBE4A is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_004788   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q14139 |
| General description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. (provided by RefSeq) |
| Immunogen: | UBE4A (NP_004779, 974 a.a. ~ 1073 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LAERIKSLADLQQQEEETYADACDE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |