Monoclonal Anti-CRSP8 antibody produced in mouse
SIGMA/WH0009442M1 - clone 8B8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CRAP34; Anti-CRSP34; Anti-MED27; Anti-MGC11274; Anti-TRAP37; Anti-cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CRSP8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 0.3 μg/mL]
Western Blotting Western Blot analysis of CRSP8 expression in transfected 293T cell line by CRSP8 monoclonal antibody, clone 8B8. Lanes Lane 1: CRSP8 transfected lysate (35.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting CRSP8 monoclonal antibody, clone 8B8 Western Blot analysis of CRSP8 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (33.44 kDa).
ELISA Detection limit for recombinant GST tagged MED27 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8B8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC002878   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q6P2C8 |
| General description: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. (provided by RefSeq) |
| Immunogen: | CRSP8 (AAH02878, 1 a.a. ~ 70 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MRNKETLEGREKAFIAHFQDNLHSV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |