Monoclonal Anti-CRSP9 antibody produced in mouse
SIGMA/WH0009443M1 - clone 3E7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CRSP33; Anti-MED7; Anti-MGC12284; Anti-cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
Product Type: Chemical
Western Blotting Western Blot analysis of CRSP9 expression in transfected 293T cell line by CRSP9 monoclonal antibody, clone 3E7. Lanes Lane 1: CRSP9 transfected lysate (27.2 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of CRSP9 over-expressed 293 cell line, cotransfected with CRSP9 Validated Chimera RNAi. Blot probed with CRSP9 monoclonal antibody, clone 3E7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting CRSP9 monoclonal antibody, clone 3E7 Western Blot analysis of CRSP9 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (51.37 kDa).
ELISA Detection limit for recombinant GST tagged CRSP9 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC005250   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43513 |
| General description: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq) |
| Immunogen: | CRSP9 (AAH05250, 1 a.a. ~ 233 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGEPQQVSALPPPPMQYIKEYTDEN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |